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Biological Scientist II - Cell Biologist

Company: University of Florida
Location: Gainesville
Posted on: July 15, 2019

Job Description:

Biological Scientist II - Cell Biologist Apply nowJob no:****** Work type:Staff Full-Time Location:Main Campus (Gainesville, FL) Categories:Research/Scientific/Grants Department:******** - MD-BIOCHEMISTRY-GENERAL Classification Title: Biological Scientist II Job Description: The Caglayan lab in the Department of Biochemistry and Molecular Biology is currently accepting applications for a Biological Scientist II. This position will be responsible for conducting molecular biological and biochemical experiments including cell viability and DNA repair assays of cultured mammalian cells in response to DNA damage inducing agents, and small-molecule inhibitors, flow cytometry-based detection of Y-H2AX, gene expression knockdown or genetic knockout experiments with CRISPR/Cas9 gene editing. Research - Formulate hypotheses to study the effect of oxidative stress on cell viability, DNA damage response, and DNA repair analysis in consultation with Principal Investigator. - Conduct and modify molecular biological methodologies, including assays for cytotoxicity of mammalian cultured cells in response to DNA damage inducing agents. - Maintain cell cultures, freezing and thawing frozen cells appropriately (included but not limited to sterile handling of reagents and cell media, checking biological contaminations, and sterile work area). Conduct experiments to analyze small-molecule inhibitors of DNA damage-repair pathways. - Perform gene expression knockdown or genetic knockout experiments. - Perform all in vivo cell viability studies using the cell counter (Beckman Vi-Cell XR) - Cultivate and maintain mammalian cell lines in controlled surroundings for study and observation and for use in experiments. - Modify existing protocols to improve existing techniques or to incorporate new procedures Search scientific literature to select and modify methods and procedures most appropriate for research goals. Conduct biological research in accordance with stipulated protocols and quality and safety standards. - Adjust experimental designs as necessary based on experimental data analysis. - Consult with researchers and other professionals to conduct appropriate analyses, analyze problems, determine strategies, or recommend solutions. - Maintain accurate laboratory records that record research methods, procedures, and results; - Prepare and maintain research findings, summaries, logs, and notes. Lab Management - Responsible for managing daily operations of the cell culture laboratory. Advise and direct the development and implementation of policies and procedures in the lab. Establish standard operating procedures and protocols for sample preparation. - Order and maintain laboratory supplies. Maintain proper records and detailed written sample preparation protocols. - Provide maintenance and culturing of cell lines. Perform routine maintenance on laboratory equipment; verify that equipment and instrumentation are maintained in working condition to ensure accuracy and quality of experimental results. - Isolate and maintain cultures of cell lines for study in prescribed or developed media, controlling moisture, aeration, temperature, and nutrition. Prepare samples and send to other investigators to fulfill reagent requests. - Maintain laboratory safety programs and training personnel in laboratory safety techniques. Assist with preparation of Institutional Review Board protocols, applications, and reports. Administrative - Provide input for technical reports, research papers, scholarly articles, abstracts, or recommendations. Preparation of figures for presentations, publications, or grants. Assist in preparation of progress reports to funding agencies. Training - Assist in teaching or instructing postdoctorals and graduate students in methods or procedures or supervising their research. Assist in managing laboratory teams or directing the work of other scientists, technicians, and other workers. This is a temporary TEAMS position, one-year appointment with possibility of extension. Advertised Salary: $38,000-$42,000 annually; Commensurate with education and experience. Minimum Requirements: Doctoral degree in biology or a closely related field; or master's degree in biology or a closely related field and one year of experience in the field or in a related area; or bachelor's degree in biology or a closely related field and three years of experience in the field or in a related area. Preferred Qualifications: - Doctoral degree in biology, molecular biology, or biochemistry; - Strong experimental background with evidence of experimental skills related to cell and molecular biology; - Experience in culture of mammalian cells (mouse embryonic fibroblast, HeLa cells); - Extensive experience in cell viability assays and treatment of cultured cells with DNA damage-inducing agents and co-treatment with small molecule inhibitors; - Experience in flow cytometry-based detection of Y-H2AX and CRISPR/Cas9 gene editing; - Experience in writing and adhering to procedures and protocols is critical; - Positive attitude to work with a track record of working well with other lab members and performing all work in a safe manner. Special Instructions to Applicants: In order to be considered, you must submit a cover letter, resume and list of at three professional references. This is a time-limited position, one-year appointment with possibility of extension. Application must be submitted by 11:55 p.m. (ET) of the posting end date. Health Assessment Required:No Advertised:08 Jul 2019Eastern Daylight Time Applications close:22 Jul 2019Eastern Daylight Time

Keywords: University of Florida, Gainesville , Biological Scientist II - Cell Biologist, Other , Gainesville, Florida

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